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random hexamer primers  (Qiagen)


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    Structured Review

    Qiagen random hexamer primers
    Random Hexamer Primers, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 2505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+hexamer+primers/Random+Hexamers/pm42068851-57-30-35
    Average 97 stars, based on 2505 article reviews
    random hexamer primers - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Generation of a human induced pluripotent stem cell line (iPSC) from a patient with Clark-Baraitser Syndrome.
    Article Snippet: .. RNA concentration and purity (A260/A280) were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of RNA using Superscript IV reverse transcriptase (Invitrogen, #18090010) and random hexamer primers (50 ng/μL; Qiagen, #79236) following the manufacturer’s instructions. .. PCR reactions were performed using Phire Hot Start II DNA polymerase (ThermoFisher) with Phire Buffer, 10 mM dNTPs (ThermoFisher), the primers of interest (Table 2), and 1 μL of cDNA.

    Article Title: Generation of two human induced pluripotent stem cell lines (iPSC) from patients with Hao-Fountain Syndrome.
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC-H2O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer’s protocol. .. Phire Hot Start II DNA polymerase (ThermoFisher) was used for PCR analysis, which was mixed with Phire Buffer (ThermoFisher), dNTPs (10 mM; ThermoFisher), the primer set of interest and 1 μl of cDNA.

    Article Title: Altered lipid profile and reduced neuronal support in human induced pluripotent stem cell ‐derived astrocytes from adrenoleukodystrophy patients
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC‐H 2 O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer's protocol. .. For quantitative PCR (qPCR) analysis, cDNA samples were diluted tenfold and run on a Lightcycler 480 (Roche) using a Sensifast SYBR Hi‐ROX‐kit (Bioline, #BIO‐92020) according to the manufacturer's protocol.

    Article Title: Altered lipid profile and reduced neuronal support in human induced pluripotent stem cell-derived astrocytes from adrenoleukodystrophy patients.
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC-H2O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer's protocol. .. For quantitative PCR (qPCR) analysis, cDNA samples were diluted tenfold and run on a Lightcycler 480 (Roche) using a Sensifast SYBR Hi-ROX-kit (Bioline, #BIO-92020) according to the manufacturer's protocol.

    Spectrophotometry:

    Article Title: Generation of a human induced pluripotent stem cell line (iPSC) from a patient with Clark-Baraitser Syndrome.
    Article Snippet: .. RNA concentration and purity (A260/A280) were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of RNA using Superscript IV reverse transcriptase (Invitrogen, #18090010) and random hexamer primers (50 ng/μL; Qiagen, #79236) following the manufacturer’s instructions. .. PCR reactions were performed using Phire Hot Start II DNA polymerase (ThermoFisher) with Phire Buffer, 10 mM dNTPs (ThermoFisher), the primers of interest (Table 2), and 1 μL of cDNA.

    Article Title: Generation of two human induced pluripotent stem cell lines (iPSC) from patients with Hao-Fountain Syndrome.
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC-H2O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer’s protocol. .. Phire Hot Start II DNA polymerase (ThermoFisher) was used for PCR analysis, which was mixed with Phire Buffer (ThermoFisher), dNTPs (10 mM; ThermoFisher), the primer set of interest and 1 μl of cDNA.

    Article Title: Altered lipid profile and reduced neuronal support in human induced pluripotent stem cell ‐derived astrocytes from adrenoleukodystrophy patients
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC‐H 2 O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer's protocol. .. For quantitative PCR (qPCR) analysis, cDNA samples were diluted tenfold and run on a Lightcycler 480 (Roche) using a Sensifast SYBR Hi‐ROX‐kit (Bioline, #BIO‐92020) according to the manufacturer's protocol.

    Article Title: Altered lipid profile and reduced neuronal support in human induced pluripotent stem cell-derived astrocytes from adrenoleukodystrophy patients.
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC-H2O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer's protocol. .. For quantitative PCR (qPCR) analysis, cDNA samples were diluted tenfold and run on a Lightcycler 480 (Roche) using a Sensifast SYBR Hi-ROX-kit (Bioline, #BIO-92020) according to the manufacturer's protocol.

    Synthesized:

    Article Title: Generation of a human induced pluripotent stem cell line (iPSC) from a patient with Clark-Baraitser Syndrome.
    Article Snippet: .. RNA concentration and purity (A260/A280) were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of RNA using Superscript IV reverse transcriptase (Invitrogen, #18090010) and random hexamer primers (50 ng/μL; Qiagen, #79236) following the manufacturer’s instructions. .. PCR reactions were performed using Phire Hot Start II DNA polymerase (ThermoFisher) with Phire Buffer, 10 mM dNTPs (ThermoFisher), the primers of interest (Table 2), and 1 μL of cDNA.

    Reverse Transcription:

    Article Title: Generation of a human induced pluripotent stem cell line (iPSC) from a patient with Clark-Baraitser Syndrome.
    Article Snippet: .. RNA concentration and purity (A260/A280) were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of RNA using Superscript IV reverse transcriptase (Invitrogen, #18090010) and random hexamer primers (50 ng/μL; Qiagen, #79236) following the manufacturer’s instructions. .. PCR reactions were performed using Phire Hot Start II DNA polymerase (ThermoFisher) with Phire Buffer, 10 mM dNTPs (ThermoFisher), the primers of interest (Table 2), and 1 μL of cDNA.

    Article Title: Proximity labeling of host factor ANXA3 in HCV infection reveals a novel LARP1 function in viral entry.
    Article Snippet: .. RNA was reversely transcribed with Superscript III reverse transcriptase (Invitrogen), random hexamer primers (Qiagen), and RNAseOut (Invitrogen). .. For qRT-PCR analysis we used Maxima SYBR Green Mastermix (Thermo Fisher) or Luna Universal qPCR Mastermix (NEB) on a 7500HT Fast Realtime PCR System or a StepOne Plus Real Time qPCR System (Applied Biosystems). qRTPCR primers were selected from the Harvard primer bank (80).

    Article Title: Dengue virus is particularly sensitive to interference with long-chain fatty acid elongation and desaturation.
    Article Snippet: Cellular DNA was digested with rDNaseI using the TURBO DNA-free DNase kit (Ambion). .. For reverse transcription into cDNA we used random hexamer primers (Qiagen), SuperScript III reverse transcriptase (Thermo Fisher), and RNaseOut (Thermo Fisher). qPCR was performed with Luna Universal qPCR master mix (New England Biolabs). ..

    Article Title: Inhibition of sterol O-acyltransferase 1 blocks Zika virus infection in cell lines and cerebral organoids
    Article Snippet: Organoid samples were vortexed with Lysing Matrix H (MPbio) in TriReagent until the tissue was completely dissolved and glycogen (RNA grade, Thermo Fisher) was added as carrier. .. Residual DNA was removed by rDNAseI-treatment (DNA-free Kit, Invitrogen), and RNA was reversely transcribed using Superscript III reverse transcriptase (Invitrogen), RNAseOut (Invitrogen), and random hexamer primers (Qiagen). qRT-PCR analysis was performed using the Luna Universal qPCR Master Mix (NEB) on a StepOne Plus Real time PCR system or a Quant Studio 3 (Applied Biosystems). ..

    Random Hexamer:

    Article Title: Generation of a human induced pluripotent stem cell line (iPSC) from a patient with Clark-Baraitser Syndrome.
    Article Snippet: .. RNA concentration and purity (A260/A280) were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of RNA using Superscript IV reverse transcriptase (Invitrogen, #18090010) and random hexamer primers (50 ng/μL; Qiagen, #79236) following the manufacturer’s instructions. .. PCR reactions were performed using Phire Hot Start II DNA polymerase (ThermoFisher) with Phire Buffer, 10 mM dNTPs (ThermoFisher), the primers of interest (Table 2), and 1 μL of cDNA.

    Article Title: Generation of two human induced pluripotent stem cell lines (iPSC) from patients with Hao-Fountain Syndrome.
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC-H2O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer’s protocol. .. Phire Hot Start II DNA polymerase (ThermoFisher) was used for PCR analysis, which was mixed with Phire Buffer (ThermoFisher), dNTPs (10 mM; ThermoFisher), the primer set of interest and 1 μl of cDNA.

    Article Title: Altered lipid profile and reduced neuronal support in human induced pluripotent stem cell ‐derived astrocytes from adrenoleukodystrophy patients
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC‐H 2 O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer's protocol. .. For quantitative PCR (qPCR) analysis, cDNA samples were diluted tenfold and run on a Lightcycler 480 (Roche) using a Sensifast SYBR Hi‐ROX‐kit (Bioline, #BIO‐92020) according to the manufacturer's protocol.

    Article Title: Proximity labeling of host factor ANXA3 in HCV infection reveals a novel LARP1 function in viral entry.
    Article Snippet: .. RNA was reversely transcribed with Superscript III reverse transcriptase (Invitrogen), random hexamer primers (Qiagen), and RNAseOut (Invitrogen). .. For qRT-PCR analysis we used Maxima SYBR Green Mastermix (Thermo Fisher) or Luna Universal qPCR Mastermix (NEB) on a 7500HT Fast Realtime PCR System or a StepOne Plus Real Time qPCR System (Applied Biosystems). qRTPCR primers were selected from the Harvard primer bank (80).

    Article Title: Altered lipid profile and reduced neuronal support in human induced pluripotent stem cell-derived astrocytes from adrenoleukodystrophy patients.
    Article Snippet: .. Samples were dissolved in 10–30 μL DMPC-H2O, and RNA concentration and 260/280 ratios were measured with a NanoDrop spectrophotometer (NanoDrop 2000 Thermo Scientific). cDNA was made from 1 μg RNA using Superscript IV (Invitrogen, #18090010) and 50 ng/μL random hexamer primers (Qiagen, #79236) according to the manufacturer's protocol. .. For quantitative PCR (qPCR) analysis, cDNA samples were diluted tenfold and run on a Lightcycler 480 (Roche) using a Sensifast SYBR Hi-ROX-kit (Bioline, #BIO-92020) according to the manufacturer's protocol.

    Article Title: Dengue virus is particularly sensitive to interference with long-chain fatty acid elongation and desaturation.
    Article Snippet: Cellular DNA was digested with rDNaseI using the TURBO DNA-free DNase kit (Ambion). .. For reverse transcription into cDNA we used random hexamer primers (Qiagen), SuperScript III reverse transcriptase (Thermo Fisher), and RNaseOut (Thermo Fisher). qPCR was performed with Luna Universal qPCR master mix (New England Biolabs). ..

    Article Title: Inhibition of sterol O-acyltransferase 1 blocks Zika virus infection in cell lines and cerebral organoids
    Article Snippet: Organoid samples were vortexed with Lysing Matrix H (MPbio) in TriReagent until the tissue was completely dissolved and glycogen (RNA grade, Thermo Fisher) was added as carrier. .. Residual DNA was removed by rDNAseI-treatment (DNA-free Kit, Invitrogen), and RNA was reversely transcribed using Superscript III reverse transcriptase (Invitrogen), RNAseOut (Invitrogen), and random hexamer primers (Qiagen). qRT-PCR analysis was performed using the Luna Universal qPCR Master Mix (NEB) on a StepOne Plus Real time PCR system or a Quant Studio 3 (Applied Biosystems). ..

    Real-time Polymerase Chain Reaction:

    Article Title: NOTCH4 Intron 28 Regulatory Element Controls Arterial Specification and Lymphoid Development from hPSCs
    Article Snippet: Luciferase activity was measured using a 9 Dual-Glo Luciferase assay kit (Promega) and SpectraMaxi3x microplate reader (Molecular Devices). .. RNA was extracted using the RNeasy Micro Kit (Qiagen) and reverse-transcribed into cDNA using random hexamer primers (Qiagen) with SMART MMLV reverse transcriptase (TaKaRa). qPCR was conducted using the SYBR Advantage qPCR Premix (TaKaRa). ..

    Article Title: Dengue virus is particularly sensitive to interference with long-chain fatty acid elongation and desaturation.
    Article Snippet: Cellular DNA was digested with rDNaseI using the TURBO DNA-free DNase kit (Ambion). .. For reverse transcription into cDNA we used random hexamer primers (Qiagen), SuperScript III reverse transcriptase (Thermo Fisher), and RNaseOut (Thermo Fisher). qPCR was performed with Luna Universal qPCR master mix (New England Biolabs). ..

    Article Title: Inhibition of sterol O-acyltransferase 1 blocks Zika virus infection in cell lines and cerebral organoids
    Article Snippet: Organoid samples were vortexed with Lysing Matrix H (MPbio) in TriReagent until the tissue was completely dissolved and glycogen (RNA grade, Thermo Fisher) was added as carrier. .. Residual DNA was removed by rDNAseI-treatment (DNA-free Kit, Invitrogen), and RNA was reversely transcribed using Superscript III reverse transcriptase (Invitrogen), RNAseOut (Invitrogen), and random hexamer primers (Qiagen). qRT-PCR analysis was performed using the Luna Universal qPCR Master Mix (NEB) on a StepOne Plus Real time PCR system or a Quant Studio 3 (Applied Biosystems). ..

    Quantitative RT-PCR:

    Article Title: Inhibition of sterol O-acyltransferase 1 blocks Zika virus infection in cell lines and cerebral organoids
    Article Snippet: Organoid samples were vortexed with Lysing Matrix H (MPbio) in TriReagent until the tissue was completely dissolved and glycogen (RNA grade, Thermo Fisher) was added as carrier. .. Residual DNA was removed by rDNAseI-treatment (DNA-free Kit, Invitrogen), and RNA was reversely transcribed using Superscript III reverse transcriptase (Invitrogen), RNAseOut (Invitrogen), and random hexamer primers (Qiagen). qRT-PCR analysis was performed using the Luna Universal qPCR Master Mix (NEB) on a StepOne Plus Real time PCR system or a Quant Studio 3 (Applied Biosystems). ..



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